Photoinduced transformation of UVR8 monitored by vibrational and fluorescence spectroscopy

Heilmann, M., Christie, J. M. , Kennis, J. T.M., Jenkins, G. I. and Mathes, T. (2015) Photoinduced transformation of UVR8 monitored by vibrational and fluorescence spectroscopy. Photochemical and Photobiological Sciences, 14(2), pp. 252-257. (doi:10.1039/c4pp00246f) (PMID:25274012)

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Abstract

Tryptophan residues at the dimer interface of the plant photoreceptor UVR8 promote monomerisation after UV-B absorption via a so far unknown mechanism. Using FTIR spectroscopy we assign light-induced structural transitions of UVR8 mainly to amino acid side chains without major transformations of the secondary structure of the physiologically relevant C-terminal extension. Additionally, we assign the monomerisation associated increase and red shift of the UVR8 tryptophan emission to a photoinduced rearrangement of tryptophan side chains and a relocation of the aspartic acid residues D96 and D107, respectively. By illumination dependent emission spectroscopy we furthermore determined the quantum yield of photoinduced monomerisation to 20 ± 8%.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Heilmann, Miss Monika and Jenkins, Professor Gareth and Christie, Professor John
Authors: Heilmann, M., Christie, J. M., Kennis, J. T.M., Jenkins, G. I., and Mathes, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Photochemical and Photobiological Sciences
Publisher:Royal Society of Chemistry
ISSN:1474-905X
ISSN (Online):1474-9092

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