Improving the detection of IgM antibodies against glycolipids complexes of GM1 and galactocerebroside in multifocal motor neuropathy using glycoarray and ELISA assays

Delmont, E., Halstead, S., Galban-Horcajo, F., Yao, D., Desnuelle, C. and Willison, H. (2015) Improving the detection of IgM antibodies against glycolipids complexes of GM1 and galactocerebroside in multifocal motor neuropathy using glycoarray and ELISA assays. Journal of Neuroimmunology, 278, pp. 159-161. (doi:10.1016/j.jneuroim.2014.11.001) (PMID:25468269)

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Abstract

Antibodies against complexes of GM1:GalC are detected in multifocal motor neuropathy. Previous studies used different techniques, explaining disparities in the results. Antibodies against GM1 and GM1:GalC with different proportions of GalC were measured with both glycoarray and ELISA in 20 multifocal motor neuropathies, and 45 controls. The 1:5 ratio and the 1:1 ratio of GM1:GalC (weight ratio) were respectively the most effective for glycoarray and for ELISA. Testing for anti-GM1:GalC antibodies increased the sensitivity from 40% with anti-GM1 antibodies to 65% with array and 60% with ELISA without loss in specificity (above 91%). Anti-GM1:GalC antibodies are effective biological tools to diagnose multifocal motor neuropathy.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Halstead, Dr Susan and Yao, Dr Denggao and Willison, Professor Hugh and Galban, Mr Francesc and Delmont, Dr Emilien
Authors: Delmont, E., Halstead, S., Galban-Horcajo, F., Yao, D., Desnuelle, C., and Willison, H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Neuroimmunology
Publisher:Elsevier
ISSN:0165-5728
ISSN (Online):1872-8421

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