Alneberg, J., Bjarnason, B. S., de Bruijn, I., Schirmer, M., Quick, J., Ijaz, U. Z. , Lahti, L., Loman, N. J., Andersson, A. F. and Quince, C. (2014) Binning metagenomic contigs by coverage and composition. Nature Methods, 11(11), pp. 1144-1146. (doi: 10.1038/NMETH.3103) (PMID:25218180)
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Abstract
Shotgun sequencing enables the reconstruction of genomes from complex microbial communities, but because assembly does not reconstruct entire genomes, it is necessary to bin genome fragments. Here we present CONCOCT, a new algorithm that combines sequence composition and coverage across multiple samples, to automatically cluster contigs into genomes. We demonstrate high recall and precision on artificial as well as real human gut metagenome data sets.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Schirmer, Ms Melanie and Quince, Dr Christopher and Ijaz, Dr Umer |
| Authors: | Alneberg, J., Bjarnason, B. S., de Bruijn, I., Schirmer, M., Quick, J., Ijaz, U. Z., Lahti, L., Loman, N. J., Andersson, A. F., and Quince, C. |
| College/School: | College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | Nature Methods |
| Publisher: | Nature Publishing Group |
| ISSN: | 1548-7091 |
| ISSN (Online): | 1548-7105 |
| Related URLs: |
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