Optoelectronic cell lysis

Neale, S. L. , Witte, C., Clark, A. W. , Reboud, J. and Cooper, J. M. (2014) Optoelectronic cell lysis. In: Optical Trapping and Optical Micromanipulation XI, San Diego, CA, USA, 17-21 Aug 2014, p. 916425. (doi:10.1117/12.2062186)

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Abstract

In this paper we show how the electrical lysis of cells can be controlled through the use of an optoelectronic device and describe how the lysis is affected by the size and shape of the cell as well as the geometry of the device and the light patterns used. By selectively illuminating a photoconductor “virtual electrodes” are created allowing the precise control of electrical fields onto the cell within the device, electrically rupturing their membranes and allowing access to intracellular contents. We examine this optically controlled electrical lysis method and discuss its advantages as a sample preparation technique. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Item Type:Conference Proceedings
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Clark, Dr Alasdair and Cooper, Professor Jonathan and Reboud, Dr Julien and Witte, Dr Christian and Neale, Dr Steven
Authors: Neale, S. L., Witte, C., Clark, A. W., Reboud, J., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
ISSN:0277-786X

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
506171Micro-Actuators Controlled by Optoelectronic Tweezers (MACOET)Jonathan CooperThe Royal Academy of Engineering (RAE)EP/G058393/1ENG - BIOMEDICAL ENGINEERING
561421Plasmon Enhanced Pyroelectric Nanoscale Trapping and SensingAlasdair ClarkThe Royal Academy of Engineering (RAE)10216/103ENG - BIOMEDICAL ENGINEERING
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING
553521Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and WellbeingJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/I017887/1ENG - BIOMEDICAL ENGINEERING