mik1+ G1-S transcription regulates mitotic entry in fission yeast

Ng, S.S., Anderson, M., White, S. and McInerny, C.J. (2001) mik1+ G1-S transcription regulates mitotic entry in fission yeast. FEBS Letters, 503(2/3), pp. 131-134. (doi: 10.1016/S0014-5793(01)02720-X)

Full text not currently available from Enlighten.

Abstract

In the fission yeast Schizosaccharomyces pombe Mik1p, in combination with Wee1p, is an important inhibitor of mitosis through direct phosphorylation of Cdc2p. Here we present the observation that mik1<sup>+</sup> is transcribed during G1- and S-phase in normally dividing cells. mik1<sup>+</sup> transcription is regulated by the MCB–DSC1 system, which controls expression of other genes at the G1–S interval. mik1<sup>+</sup> is shown to be an important target of MCB–DSC1 as it is epistatic for the mitotic delay phenotype displayed in cdc10-C4 cells, which are mutated in a component of DSC1. The mitotic delay in cdc10-C4 cells is bypassed by cdc2-1w, suggesting that mik1<sup>+</sup> acts directly on cdc2<sup>+</sup>, with no checkpoint function involved. Thus, mik1<sup>+</sup> represents a new type of MCB–DSC1 regulated gene in fission yeast, whose gene product is exclusively expressed during G1- and S-phase to prevent premature mitosis during this cell cycle stage.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInerny, Dr Chris and White, Dr Stewart
Authors: Ng, S.S., Anderson, M., White, S., and McInerny, C.J.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:FEBS Letters
ISSN:0014-5793
ISSN (Online):1873-3468

University Staff: Request a correction | Enlighten Editors: Update this record