mik1+ G1-S transcription regulates mitotic entry in fission yeast

Ng, S.S., Anderson, M., White, S. and McInerny, C.J. (2001) mik1+ G1-S transcription regulates mitotic entry in fission yeast. FEBS Letters, 503(2/3), pp. 131-134. (doi: 10.1016/S0014-5793(01)02720-X)

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In the fission yeast Schizosaccharomyces pombe Mik1p, in combination with Wee1p, is an important inhibitor of mitosis through direct phosphorylation of Cdc2p. Here we present the observation that mik1<sup>+</sup> is transcribed during G1- and S-phase in normally dividing cells. mik1<sup>+</sup> transcription is regulated by the MCB–DSC1 system, which controls expression of other genes at the G1–S interval. mik1<sup>+</sup> is shown to be an important target of MCB–DSC1 as it is epistatic for the mitotic delay phenotype displayed in cdc10-C4 cells, which are mutated in a component of DSC1. The mitotic delay in cdc10-C4 cells is bypassed by cdc2-1w, suggesting that mik1<sup>+</sup> acts directly on cdc2<sup>+</sup>, with no checkpoint function involved. Thus, mik1<sup>+</sup> represents a new type of MCB–DSC1 regulated gene in fission yeast, whose gene product is exclusively expressed during G1- and S-phase to prevent premature mitosis during this cell cycle stage.

Item Type:Articles
Glasgow Author(s) Enlighten ID:McInerny, Dr Chris and White, Dr Stewart
Authors: Ng, S.S., Anderson, M., White, S., and McInerny, C.J.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:FEBS Letters
ISSN (Online):1873-3468

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