Baldini, F., Chiavaioli, F., Giannetti, A., Brenci, M. and Trono, C. (2013) Label-free biosensor based on long period grating. In: Advanced Biomedical and Clinical Diagnostic Systems XI, San Francisco, C.A., USA, 2 Feb 2013, (doi: 10.1117/12.2007399)
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Abstract
Long period gratings have been recently proposed as label-free optical devices for biochemical sensing. A biochemical interaction along the grating region changes the biolayer refractive index and a change in the fiber transmission spectrum occurs. The fiber biofunctionalization was performed with a novel chemistry using Eudragit L100 copolymer as opposed to the commonly-used silanization procedure. An IgG/anti-IgG bioassay was carried out for studying the antigen/antibody interaction. The biosensor was fully characterized, monitoring the kinetics during the antibody immobilization and achieving the calibration curve of the assay. To compare the biosensor performance, two LPG-based biosensors with distinct grating periods were characterized following the same bioassay protocol. Experimental results demonstrated an enhancement of the biosensor performance when the fundamental core mode of a single-mode fiber couples with a higher order cladding mode. Considering an LPG manufactured on a bare optical fiber, in which the coupling occurs with the 7-th cladding mode, a dynamic signal range of 0.33 nm, a working range of 1.7 – 1450 mg L-1 and a LOD of 500 μg L-1 were achieved.
Item Type: | Conference Proceedings |
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Additional Information: | Proceedings of SPIE : 8572. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | UNSPECIFIED |
Authors: | Baldini, F., Chiavaioli, F., Giannetti, A., Brenci, M., and Trono, C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
ISSN: | 0277-786X |
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