Label-free biosensor based on long period grating

Baldini, F., Chiavaioli, F., Giannetti, A., Brenci, M. and Trono, C. (2013) Label-free biosensor based on long period grating. In: Advanced Biomedical and Clinical Diagnostic Systems XI, San Francisco, C.A., USA, 2 Feb 2013, (doi: 10.1117/12.2007399)

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Abstract

Long period gratings have been recently proposed as label-free optical devices for biochemical sensing. A biochemical interaction along the grating region changes the biolayer refractive index and a change in the fiber transmission spectrum occurs. The fiber biofunctionalization was performed with a novel chemistry using Eudragit L100 copolymer as opposed to the commonly-used silanization procedure. An IgG/anti-IgG bioassay was carried out for studying the antigen/antibody interaction. The biosensor was fully characterized, monitoring the kinetics during the antibody immobilization and achieving the calibration curve of the assay. To compare the biosensor performance, two LPG-based biosensors with distinct grating periods were characterized following the same bioassay protocol. Experimental results demonstrated an enhancement of the biosensor performance when the fundamental core mode of a single-mode fiber couples with a higher order cladding mode. Considering an LPG manufactured on a bare optical fiber, in which the coupling occurs with the 7-th cladding mode, a dynamic signal range of 0.33 nm, a working range of 1.7 – 1450 mg L-1 and a LOD of 500 μg L-1 were achieved.

Item Type:Conference Proceedings
Additional Information:Proceedings of SPIE : 8572.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:UNSPECIFIED
Authors: Baldini, F., Chiavaioli, F., Giannetti, A., Brenci, M., and Trono, C.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
ISSN:0277-786X

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