Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases

Xu, G., Gunson, R. N., Cooper, J. and Reboud, J. (2015) Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases. Chemical Communications, 51(13), pp. 2589-2592. (doi:10.1039/c4cc08389j) (PMID:25569801)

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Abstract

We describe a nucleic acid testing (NAT) platform for infectious disease diagnostics at the point-of-care, using surface acoustic waves (SAW) to perform a multiplexed loop-mediated isothermal amplification (LAMP) test for sexually transmitted diseases. The ultrasonic actuation not only enables faster NAT reactions but also provides a route towards integrating low-cost, low-power molecular diagnostics into disposable sensors.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gunson, Dr Rory and Cooper, Professor Jonathan and Reboud, Dr Julien
Authors: Xu, G., Gunson, R. N., Cooper, J., and Reboud, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Chemical Communications
Publisher:R S C Publications
ISSN:1359-7345
ISSN (Online):0009-241X
Copyright Holders:Copyright © 2015 The Royal Society of Chemistry
First Published:First published in Chemical Communications 51(13):2589-2592
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING