Principles of tissue culture and bioreactor design

Freshney, R.I., Obradovic, B., Grayson, W., Cannizzaro, C. and Vunjak-Novakovic, G. (2007) Principles of tissue culture and bioreactor design. In: Lanza, R., Langer, R. and Vacanti, J. (eds.) Principles of Tissue Engineering, 3rd Edition. Academic Press, pp. 155-183. ISBN 9780123706157 (doi: 10.1016/B978-012370615-7/50016-0)

Abstract

In this chapter, we review the principles of cell culture and bioreactor design in the context of tissue engineering. In the first part, we describe the principles of cell and tissue culture, with emphasis on the initiation of culture, transformation, preservation, and validation of the cells and differentiation methods that utilize simple and well-established petri dish protocols. In the second part, we describe the design and operation of tissue engineering bioreactors, with focus on mass transport considerations associated with environmental control, and the provision of biophysical signals associated with the modulation of cell differentiation and functional tissue assembly. We provide eight examples of representative bioreactor designs that illustrate how some of the differentiation factors are provided in a physiologically relevant fashion.

• Item Type: Book Sections
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Freshney, Dr Ian
• Authors: Freshney, R.I., Obradovic, B., Grayson, W., Cannizzaro, C., and Vunjak-Novakovic, G.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Principles of Tissue Engineering
• Publisher: Academic Press
• ISBN: 9780123706157