Mycobacterium bovis-BCG vaccination induces specific pulmonary transcriptome biosignatures in mice

Aranday-Cortes, E. , Kaveh, D., Nunez-Garcia, J., Hogarth, P. J. and Vordermeier, H. M. (2010) Mycobacterium bovis-BCG vaccination induces specific pulmonary transcriptome biosignatures in mice. PLoS ONE, 5(6), e11319. 20596522. (doi: 10.1371/journal.pone.0011319) (PMID:PMC2893133)

103658.pdf - Published Version



Background: In the present study, we applied microarray technology to define biosignatures by microarray transcriptome analysis in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before challenge, and biomarker patterns that correlated with anamnestic protective responses following exposure to virulent M. bovis. Further, these biosignatures should be detectable without in vitro antigenic challenge.<p></p> Principal Findings: After BCG vaccination, we defined a specific pulmonary gene expression signature related to the connective tissue development and function network that predicted vaccine success before M. bovis challenge. In addition, a Th17-related cytokine profile was found that correlated with vaccine-induced protective immunity following infection with virulent M. bovis in the lung as well as additional genes that were up-regulated in the spleens of vaccinated animals post-infection related to neutrophil biology and inflammation.<p></p> Conclusions: This study has therefore prioritized both biomarkers predicting vaccination success before challenge and bio-signatures that are potentially associated with protective immune responses that will be useful to evaluate future vaccine candidates.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Aranday-Cortes, Dr Elihu
Authors: Aranday-Cortes, E., Kaveh, D., Nunez-Garcia, J., Hogarth, P. J., and Vordermeier, H. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2010 Crown
First Published:First published in PLoS One 5(6):e11319
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record