Nanotopology potentiates growth hormone signalling and osteogenesis of mesenchymal stem cells

Wang, J. R., Ahmed, S. F., Gadegaard, N. , Meek, R.M. D., Dalby, M. J. and Yarwood, S. J. (2014) Nanotopology potentiates growth hormone signalling and osteogenesis of mesenchymal stem cells. Growth Hormone and IGF Research, 24(6), pp. 245-250. (doi:10.1016/j.ghir.2014.10.003) (PMID:25466909)

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Abstract

Custom engineered materials can influence the differentiation of human mesenchymal stem cells (MSCs) towards osteoblasts, chondrocytes and adipocytes, through the control of chemistry, stiffness and nanoscale topography. Here we demonstrate that polycaprolactone growth surfaces engineered with disordered (but controlled) 120 nm diameter dots (NSQ50), but not flat surfaces, promote osteogenic conversion of MSCs in the absence of other osteogenic stimuli. Differentiating MSCs on NSQ50 were found to express growth hormone receptors (GH) and stimulation with recombinant human GH (rhGH) further enhanced NSQ50-driven osteogenic conversion of MSCs. This increased osteogenesis coincided with an enhanced ability of GH to activate ERK MAP kinase on NSQ50, but not on flat topology. The importance of ERK for MSC differentiation was demonstrated by using the inhibitor of ERK activation, U0126, which completely suppressed osteogenesis of GH-stimulated MSCs on NSQ50. The ability of GH to activate ERK in MSCs may therefore be a central control mechanism underlying bone development and growth.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yarwood, Dr Stephen and Dalby, Professor Matthew and Gadegaard, Professor Nikolaj and Ahmed, Professor Syed Faisal
Authors: Wang, J. R., Ahmed, S. F., Gadegaard, N., Meek, R.M. D., Dalby, M. J., and Yarwood, S. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Growth Hormone and IGF Research
Publisher:Elsevier
ISSN:1096-6374
ISSN (Online):1096-6374

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
608751Dynamic surfaces to mimic mesenchymal stem cell niche functionsMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006908/1RI MOLECULAR CELL & SYSTEMS BIOLOGY