Evidence of natural Wolbachia infections in field populations of Anopheles gambiae

Baldini, F., Segata, N., Pompon, J., Marcenac, P., Shaw, W. R., Dabiré, R. K., Diabaté, A., Levashina, E. A. and Catteruccia, F. (2014) Evidence of natural Wolbachia infections in field populations of Anopheles gambiae. Nature Communications, 5, 3985. (doi:10.1038/ncomms4985) (PMID:24905191) (PMCID:PMC4059924)

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Abstract

Wolbachia are maternally transmitted intracellular bacteria that invade insect populations by manipulating their reproduction and immunity and thus limiting the spread of numerous human pathogens. Experimental Wolbachia infections can reduce Plasmodium numbers in Anopheles mosquitoes in the laboratory, however, natural Wolbachia infections in field anophelines have never been reported. Here we show evidence of Wolbachia infections in Anopheles gambiae in Burkina Faso, West Africa. Sequencing of the 16S rRNA gene identified Wolbachia sequences in both female and male germlines across two seasons, and determined that these sequences are vertically transmitted from mother to offspring. Whole-genome sequencing of positive samples suggests that the genetic material identified in An. gambiae belongs to a novel Wolbachia strain, related to but distinct from strains infecting other arthropods. The evidence of Wolbachia infections in natural Anopheles populations promotes further investigations on the possible use of natural Wolbachia–Anopheles associations to limit malaria transmission.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Baldini, Dr Francesco
Authors: Baldini, F., Segata, N., Pompon, J., Marcenac, P., Shaw, W. R., Dabiré, R. K., Diabaté, A., Levashina, E. A., and Catteruccia, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Nature Communications
Publisher:Nature Publishing Group
ISSN:2041-1723
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2014 Macmillan Publishers Limited
First Published:First published in Nature Communications 5:3985
Publisher Policy:Reproduced under a Creative Commons License

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