An integrated microspectrometer for localised multiplexing measurements

Hu, Z., Glidle, A., Ironside, C., Cooper, J. M. and Yin, H. (2015) An integrated microspectrometer for localised multiplexing measurements. Lab on a Chip, 15(1), pp. 283-289. (doi:10.1039/C4LC00952E)

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Publisher's URL: http://dx.doi.org/10.1039/C4LC00952E

Abstract

We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel “bead–AWG” platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing and Ironside, Professor Charles and Hu, Mr Zhixiong and Glidle, Dr Andrew and Cooper, Professor Jonathan
Authors: Hu, Z., Glidle, A., Ironside, C., Cooper, J. M., and Yin, H.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Lab on a Chip 15(1):283-289
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
532401Engineering a semi-biotic systemHuabing YinEngineering & Physical Sciences Research Council (EPSRC)EP/H04986X/1ENG - BIOMEDICAL ENGINEERING
532403Engineering a semi-biotic systemHuabing YinEngineering & Physical Sciences Research Council (EPSRC)EP/H04986X/1ENG - BIOMEDICAL ENGINEERING
574961Shell inspiration: turning nature's secrets into engineering solutions.Huabing YinEngineering & Physical Sciences Research Council (EPSRC)EP/J009121/1ENG - BIOMEDICAL ENGINEERING
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING