Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection

Watson, E., Alberdi, M. P., Inglis, N. F., Lainson, A., Porter, M. E., Manson, E., Imrie, L., Mclean, K. and Smith, D. G.E. (2014) Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection. Veterinary Microbiology, 174(3-4), pp. 448-455. (doi: 10.1016/j.vetmic.2014.10.002) (PMID:25457368)

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Abstract

Lawsonia intracellularis is the aetiological agent of the commercially significant porcine disease, proliferative enteropathy. Current understanding of host–pathogen interaction is limited due to the fastidious microaerophilic obligate intracellular nature of the bacterium. In the present study, expression of bacterial proteins during infection was investigated using a mass spectrometry approach. LC-ESI-MS/MS analysis of two isolates of L. intracellularis from heavily-infected epithelial cell cultures and database mining using fully annotated L. intracellularis genome sequences identified 19 proteins. According to the Clusters of Orthologous Groups (COG) functional classification, proteins were identified with roles in cell metabolism, protein synthesis and oxidative stress protection; seven proteins with putative or unknown function were also identified. Detailed bioinformatic analyses of five uncharacterised proteins, which were expressed by both isolates, identified domains and motifs common to other outer membrane-associated proteins with important roles in pathogenesis including adherence and invasion. Analysis of recombinant proteins on Western blots using immune sera from L. intracellularis-infected pigs identified two proteins, LI0841 and LI0902 as antigenic. The detection of five outer membrane proteins expressed during infection, including two antigenic proteins, demonstrates the potential of this approach to interrogate L. intracellularis host–pathogen interactions and identify novel targets which may be exploited in disease control.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Alberdi, Dr Maria and Smith, Professor David
Authors: Watson, E., Alberdi, M. P., Inglis, N. F., Lainson, A., Porter, M. E., Manson, E., Imrie, L., Mclean, K., and Smith, D. G.E.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Veterinary Microbiology
Publisher:Elsevier B.V.
ISSN:0378-1135
ISSN (Online):1873-2542

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
378901Molecular and functional characterisation of a type III secretion system (TTSS) of the obligate intracellular bacterium Lawsonia ....David SmithBiotechnology and Biological Sciences Research Council (BBSRC)BB/C510532/1III - BACTERIOLOGY