The sheep tetherin paralog oBST2B blocks envelope glycoprotein incorporation into nascent retroviral virions

Murphy, L. et al. (2015) The sheep tetherin paralog oBST2B blocks envelope glycoprotein incorporation into nascent retroviral virions. Journal of Virology, 89(1), pp. 535-544. (doi:10.1128/JVI.02751-14)

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Abstract

Bone marrow stromal cell antigen 2 (BST2) is a cellular restriction factor with a broad antiviral activity. In sheep, the BST2 gene is duplicated into two paralogs termed oBST2A and oBST2B. oBST2A impedes viral exit of the Jaagsiekte sheep retroviruses (JSRV), most probably by retaining virions at the cell membrane, similar to the “tethering” mechanism exerted by human BST2. In this study, we provide evidence that unlike oBST2A, oBST2B is limited to the Golgi apparatus and disrupts JSRV envelope (Env) trafficking by sequestering it. In turn, oBST2B leads to a reduction in Env incorporation into viral particles, which ultimately results in the release of virions that are less infectious. Furthermore, the activity of oBST2B does not seem to be restricted to retroviruses, as it also acts on vesicular stomatitis virus glycoproteins. Therefore, we suggest that oBST2B exerts antiviral activity using a mechanism distinct from the classical tethering restriction observed for oBST2A.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Palmarini, Professor Massimo and Murgia, Dr Claudio and Golder, Mr Matthew and Varela, Dr Mariana
Authors: Murphy, L., Varela, M., Desloire, S., Ftaich, N., Murgia, C., Golder, M., Neil, S., Spencer, T. E., Wootton, S. K., Lavillette, D., Terzian, C., Palmarini, M., and Arnaud, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virology
Publisher:American Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1098-5514

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