Analysis of non-thermal plasma-treated water using single molecule measurement of large DNA damage

Cunningham, S., Nakajima, T., Kurita, H., Yasuda, H., Takashima, K. and Mizuno, A. (2012) Analysis of non-thermal plasma-treated water using single molecule measurement of large DNA damage. International Journal of Plasma Environmental Science and Technology, 6(1), pp. 54-58.

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Publisher's URL: http://www.iesj.org/html/service/ijpest/vol6_no1_2012/ijpest_vol6_no1_2012_pp054-058.html

Abstract

An investigation to evaluate water safety regarding residual radicals as a result of water exposure to non-thermal plasma (NTP) is reported. In this investigation the kinetic of strand breakages of large DNA molecules suspended in water exposed to an argon plasma jet was studied using single molecule measurements. The single molecule measurements were also conducted on pure water after post exposure to plasma. Results show that plasma (Argon jet) exposure caused a marked reduction in length of DNA molecules suspended in water. The value of constant k- (compare the average length of DNA) was estimated using a simple mathematical model. The estimated k-value was less than 1/20 of that of the direct exposure. Data analysis show that k-value decreased by increasing the post-exposure time to plasma. Evidence of fragmentation of DNA molecules lasted at least 1 hour after plasma exposure (the longest post exposure time measured in the experiment). This direct DNA fragments analysis allows the evaluation of safety of plasma treated water effectively. It also permits the comparison between the effects of different plasma treatments on DNA rate of fragmentation. It is not clear from the data if the increase in exposure time causes an increase in the number of radicals or another mechanism is taking place.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cunningham, Dr Samia
Authors: Cunningham, S., Nakajima, T., Kurita, H., Yasuda, H., Takashima, K., and Mizuno, A.
College/School:College of Science and Engineering
Journal Name:International Journal of Plasma Environmental Science and Technology
Publisher:International Electrostatics Assembly
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in International Journal of Plasma Environmental Science and Technology 6(1):54-58
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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