Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation

Ghazali, M., Koh-Tan, H. H., McLaughlin, M. , Montague, P., Jonsson, N. N. and Eckersall, P. (2014) Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation. BMC Veterinary Research, 10(1), 204. (doi: 10.1186/s12917-014-0204-9) (PMID:25927913) (PMCID:PMC4173004)

Abstract

Background: Nasal secretion (NS) was investigated as a source of information regarding the mucosal and systemic immune status of cattle challenged by respiratory disease. A method for the collection of substantial volumes (~12 ml) of NS from cattle was developed to establish a reference range of analytes that are present in the NS of healthy cattle. Biochemical profiles of NS from a group of 38 healthy Holstein-Friesian cows revealed high alkaline phosphatase (AP) activity of up to 2392 IU/L. The character and source of the high activity of AP in bovine NS was investigated.<p></p> Results: Histochemical analysis confirmed the localization of the AP enzyme activity to epithelial cells and serous glands of the nasal respiratory mucosa. Analysis of mRNA levels from nasal mucosa by end point RT-PCR and PCR product sequencing confirmed that the AP was locally produced and is identical at the nucleotide level to the non-specific AP splice variant found in bovine liver, bone and kidney. Analysis by isoelectric focussing confirmed that AP was produced locally at a high level in nasal epithelium demonstrating that AP from nasal secretion and nasal mucosa had similar pI bands, though differing from those of the liver, kidney, bone and intestine, suggesting different post-translational modification (PTM) of AP in these tissues.<p></p> Conclusions: A nasal isozyme of AP has been identified that is present at a high activity in NS, resulting from local production and showing distinctive PTM and may be active in NS as an anti-endotoxin mediator.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Eckersall, Professor David and Ghazali, Mr Mohd and Montague, Dr Paul and McLaughlin, Dr Mark and Jonsson, Professor Nicholas and Koh-Tan, Dr Caline
• Authors: Ghazali, M., Koh-Tan, H. H., McLaughlin, M., Montague, P., Jonsson, N. N., and Eckersall, P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: BMC Veterinary Research
• Publisher: BioMed Central Ltd
• ISSN: 1746-6148
• ISSN (Online): 1746-6148
• Copyright Holders: Copyright © 2014 The Authors
• First Published: First published in BMC Veterinary Research 10(1):204
• Publisher Policy: Reproduced under a Creative Commons License