Cyr, M. M., Baatjes, A. J., Dorman, S. C., Crawford, L., Sehmi, R., Foley, R., Alam, R., O'Byrne, P. and Denburg, J. A. (2008) In Vitro effects of budesonide on eosinophil-basophil lineage commitment. Open Respiratory Medicine Journal, 2(1), pp. 60-66. (doi: 10.2174/1874306400802010060)
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Publisher's URL: http://dx.doi.org/10.2174/1874306400802010060
Abstract
IL-5 is the primary cytokine that stimulates the production and survival of eosinophils and basophils from progenitor cells. The inhaled glucocorticoid, budesonide, has been shown to exert a therapeutic effect via suppression of eosinophil/basophil progenitors in vivo. Since various steroids have exhibited the ability to enhance eosinophil/basophil progenitor differentiation, we examined the effects of budesonide in vitro. Bone marrow and cord blood samples were obtained and cultured in the presence of IL-5 alone or IL-5 plus budesonide. Eosinophil/basophil colony-forming units were enumerated from cultured nonadherent mononuclear cells and from purified CD34+ cells. CD34+ cells with and without budesonide were also examined for up-regulation of ERK1/2, MAPK and GATA-1 using real time-PCR. Results: i) upregulation of eosinophil/basophil colony-forming units is due to the direct effects of budesonide on IL-5-stimulated progenitors; ii) GATA-1 is likely involved in the early amplification of eosinophil/basophil progenitor commitment leading to increased differentiation. A potential transcriptional pathway has been identified which may mediate the effects of budesonide on eosinophil/basophil lineage commitment.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Stewart, Ms Lynn |
| Authors: | Cyr, M. M., Baatjes, A. J., Dorman, S. C., Crawford, L., Sehmi, R., Foley, R., Alam, R., O'Byrne, P., and Denburg, J. A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Open Respiratory Medicine Journal |
| Publisher: | Bentham Open |
| ISSN: | 1874-3064 |
| Copyright Holders: | Copyright © 2008 The Authors |
| First Published: | First published in Open Respiratory Medicine Journal 2(1):60-66 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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