In Vitro effects of budesonide on eosinophil-basophil lineage commitment

Cyr, M. M., Baatjes, A. J., Dorman, S. C., Crawford, L., Sehmi, R., Foley, R., Alam, R., O'Byrne, P. and Denburg, J. A. (2008) In Vitro effects of budesonide on eosinophil-basophil lineage commitment. Open Respiratory Medicine Journal, 2(1), pp. 60-66. (doi: 10.2174/1874306400802010060)

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Publisher's URL: http://dx.doi.org/10.2174/1874306400802010060

Abstract

IL-5 is the primary cytokine that stimulates the production and survival of eosinophils and basophils from progenitor cells. The inhaled glucocorticoid, budesonide, has been shown to exert a therapeutic effect via suppression of eosinophil/basophil progenitors in vivo. Since various steroids have exhibited the ability to enhance eosinophil/basophil progenitor differentiation, we examined the effects of budesonide in vitro. Bone marrow and cord blood samples were obtained and cultured in the presence of IL-5 alone or IL-5 plus budesonide. Eosinophil/basophil colony-forming units were enumerated from cultured nonadherent mononuclear cells and from purified CD34+ cells. CD34+ cells with and without budesonide were also examined for up-regulation of ERK1/2, MAPK and GATA-1 using real time-PCR. Results: i) upregulation of eosinophil/basophil colony-forming units is due to the direct effects of budesonide on IL-5-stimulated progenitors; ii) GATA-1 is likely involved in the early amplification of eosinophil/basophil progenitor commitment leading to increased differentiation. A potential transcriptional pathway has been identified which may mediate the effects of budesonide on eosinophil/basophil lineage commitment.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stewart, Ms Lynn
Authors: Cyr, M. M., Baatjes, A. J., Dorman, S. C., Crawford, L., Sehmi, R., Foley, R., Alam, R., O'Byrne, P., and Denburg, J. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Open Respiratory Medicine Journal
Publisher:Bentham Open
ISSN:1874-3064
Copyright Holders:Copyright © 2008 The Authors
First Published:First published in Open Respiratory Medicine Journal 2(1):60-66
Publisher Policy:Reproduced under a Creative Commons License
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