Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome

Haggarty, J., Oppermann, M., Dalby, M. J. , Burchmore, R. J. , Cook, K., Weidt, S. and Burgess, K. E.V. (2015) Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome. Metabolomics, 11(5), pp. 1465-1470. (doi:10.1007/s11306-014-0770-7) (PMID:26366140) (PMCID:PMC4559102)

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Abstract

The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Weidt, Dr Stefan and Burgess, Dr Karl and Haggarty, Ms Jennifer and Dalby, Professor Matthew
Authors: Haggarty, J., Oppermann, M., Dalby, M. J., Burchmore, R. J., Cook, K., Weidt, S., and Burgess, K. E.V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Metabolomics
Publisher:Springer US
ISSN:1573-3882
ISSN (Online):1573-3890
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Metabolomics 11(5): 1465-1470
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470561DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING
470563DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING