Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples

Riggio, M.P. , Macfarlane, T.W., Mackenzie, D., Lennon, A., Smith, A.J. and Kinane, D. (1996) Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples. Journal of Periodontal Research, 31(7), pp. 496-501. (doi:10.1111/j.1600-0765.1996.tb01415.x)

Full text not currently available from Enlighten.

Abstract

In this study, the major periodontal pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were detected in subgingival plaque samples from patients with periodontal disease by polymerase chain reaction (PCR) and conventional culture methods. 170 plaque samples from 43 patients were analysed; A. actinomycetemcomitans and P. gingivalis were each detected in 40 (24%) of samples by PCR, whereas conventional culture methods detected A. actinomycetemcomitans and P. gingivalis in 25 (15%) and 18 (11%) of samples, respectively. The proportion of patients carrying A. actinomycetemcomitans in at least 1 sampled periodontal site was 17/43 (40%) by PCR and 13/43 (30%) by culture; for P. gingivalis this was 12/43 (28%) by PCR and 9/43 (21%) by culture. Only 5 samples, from 3 patients, harboured both A. actinomycetemcomitans and P. gingivalis. It is concluded that PCR is more accurate than conventional culture methods for identification of these periodontal pathogens in subgingival plaque samples and has a higher frequency of detection.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lennon, Mr Alan and Riggio, Dr Marcello
Authors: Riggio, M.P., Macfarlane, T.W., Mackenzie, D., Lennon, A., Smith, A.J., and Kinane, D.
Subjects:R Medicine > RK Dentistry
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Journal of Periodontal Research
Publisher:John Wiley & Sons Ltd.
ISSN:0022-3484
ISSN (Online):1600-0765
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record