Identification and nucleotide sequence of the heat shock protein 60 (GroEL) gene of Bacteroides forsythus

Reid, H. I. and Riggio, M. P. (1998) Identification and nucleotide sequence of the heat shock protein 60 (GroEL) gene of Bacteroides forsythus. DNA Sequence: The Journal of DNA Sequencing and Mapping, 9(5-6), pp. 359-364. (doi:10.3109/10425179809008477) (PMID:10524765)

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Abstract

Bacteroides forsythus is a Gram negative anaerobe associated with human periodontal disease. Heat shock proteins are immunodominant antigens in bacteria that can elicit strong and protective immune responses. The gene specifying the GroEL protein (hsp60, heat shock protein 60) of B. forsythus was isolated by PCR amplification using consensus primers based upon published nucleotide sequences of the groEL genes of several bacterial species. Translation of the gene sequence predicts a protein of 544 amino acids in length with a molecular mass of 58 kDa. B. forsythus GroEL demonstrates identities of 50 to 81% with the predicted amino acid sequences of GroEL proteins of several bacterial species and the human mitochondrial P1 protein.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gadegaard, Dr Helen and Riggio, Dr Marcello
Authors: Reid, H. I., and Riggio, M. P.
Subjects:R Medicine > RK Dentistry
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:DNA Sequence: The Journal of DNA Sequencing and Mapping
Publisher:Informa Healthcare
ISSN:1042-5179
Published Online:11 July 2009

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