Reid, H. I. and Riggio, M. P. (1998) Identification and nucleotide sequence of the heat shock protein 60 (GroEL) gene of Bacteroides forsythus. DNA Sequence: The Journal of DNA Sequencing and Mapping, 9(5-6), pp. 359-364. (doi: 10.3109/10425179809008477) (PMID:10524765)
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Abstract
Bacteroides forsythus is a Gram negative anaerobe associated with human periodontal disease. Heat shock proteins are immunodominant antigens in bacteria that can elicit strong and protective immune responses. The gene specifying the GroEL protein (hsp60, heat shock protein 60) of B. forsythus was isolated by PCR amplification using consensus primers based upon published nucleotide sequences of the groEL genes of several bacterial species. Translation of the gene sequence predicts a protein of 544 amino acids in length with a molecular mass of 58 kDa. B. forsythus GroEL demonstrates identities of 50 to 81% with the predicted amino acid sequences of GroEL proteins of several bacterial species and the human mitochondrial P1 protein.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Gadegaard, Dr Helen and Riggio, Professor Marcello |
| Authors: | Reid, H. I., and Riggio, M. P. |
| Subjects: | R Medicine > RK Dentistry |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School College of Medical Veterinary and Life Sciences > School of Life Sciences |
| Journal Name: | DNA Sequence: The Journal of DNA Sequencing and Mapping |
| Publisher: | Informa Healthcare |
| ISSN: | 1042-5179 |
| Published Online: | 11 July 2009 |
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