Inducing experimental arthritis and breaking self-tolerance to joint-specific antigens with trackable, ovalbumin-specific T cells

Maffia, P. , Brewer, J.M. , Gracie, J.A., Ianaro, A., Leung, B.P., Mitchell, P.J., Smith, K., McInnes, I. B. and Garside, P. (2004) Inducing experimental arthritis and breaking self-tolerance to joint-specific antigens with trackable, ovalbumin-specific T cells. Journal of Immunology, 173(1), pp. 151-156. (doi:10.4049/jimmunol.173.1.151) (PMID:15210769)

Maffia, P. , Brewer, J.M. , Gracie, J.A., Ianaro, A., Leung, B.P., Mitchell, P.J., Smith, K., McInnes, I. B. and Garside, P. (2004) Inducing experimental arthritis and breaking self-tolerance to joint-specific antigens with trackable, ovalbumin-specific T cells. Journal of Immunology, 173(1), pp. 151-156. (doi:10.4049/jimmunol.173.1.151) (PMID:15210769)

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Publisher's URL: http://www.jimmunol.org/cgi/content/abstract/173/1/151

Abstract

The importance of T cell Ag specificity and Th1 vs Th2 phenotype in synovial inflammation remains controversial. Using OVA-specific TCR transgenic T cells from DO11.10 mice, we demonstrate that mice receiving Th1, but not Th2, cells display a transient arthritis following immunization that is characterized by synovial hyperplasia, cellular infiltration, and cartilage erosion. OVA-specific T cells also accumulated in inflamed joints, suggesting that they could exert their inflammatory effect locally in the joint or in the draining lymph node. Importantly, this pathology was accompanied by a breakdown in self-tolerance, as evidenced by the induction of collagen-specific T and B cell responses. This model directly demonstrates a pivotal role for Th1 cells of an irrelevant specificity in the development of inflammatory arthritis. Furthermore, the ability to track these cells in vivo will make feasible studies revealing the dynamic role of T cells in arthritis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInnes, Professor Iain and Maffia, Dr Pasquale and Garside, Professor Paul and Brewer, Professor James and Leung, Dr Bernard
Authors: Maffia, P., Brewer, J.M., Gracie, J.A., Ianaro, A., Leung, B.P., Mitchell, P.J., Smith, K., McInnes, I. B., and Garside, P.
Subjects:Q Science > QR Microbiology > QR180 Immunology
R Medicine > RC Internal medicine
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Journal of Immunology
Journal Abbr.:J. Immunol.
ISSN:0022-1767
ISSN (Online):1550-6606

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