The role of DSC1 components cdc10+, rep1+ and rep2+ in MCB gene transcription at the mitotic G1-S boundary in fission yeast

White, S., Khaliq, F., Sotiriou, S. and McInerny, C.J. (2001) The role of DSC1 components cdc10+, rep1+ and rep2+ in MCB gene transcription at the mitotic G1-S boundary in fission yeast. Current Genetics, 40(4), pp. 251-259. (doi: 10.1007/s002940100248)

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Abstract

In this paper, we describe the transcription profile of a group of genes at the G1-S boundary of fission yeast in synchronously dividing mitotic cells, under a variety of different conditions. This transcription profile is unaffected in cells where either cdc10<sup>+</sup> or cdc10-C4 are constitutively overexpressed. In contrast, overexpression of either rep1<sup>+</sup> or rep2<sup>+</sup> results in constitutive expression of MCB-regulated genes, suggesting that these polypeptides have important regulatory properties in controlling MCB transcription. Finally, we examine the pattern of MCB-regulated transcription in cells where the G1 period is extended. Surprisingly, we find that the wee1-50 mutation causes MCB transcription throughout the cell cycle, whereas cells re-fed after nitrogen starvation have normal expression patterns. The implications of these observations for understanding MCB-regulated transcription are discussed.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInerny, Dr Chris and White, Dr Stewart
Authors: White, S., Khaliq, F., Sotiriou, S., and McInerny, C.J.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Current Genetics
Journal Abbr.:Curr. Genet.
ISSN:0172-8083
ISSN (Online):1432-0983

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