The role of DSC1 components cdc10⁺, rep1⁺ and rep2⁺ in MCB gene transcription at the mitotic G1-S boundary in fission yeast

White, S., Khaliq, F., Sotiriou, S. and McInerny, C.J. (2001) The role of DSC1 components cdc10⁺, rep1⁺ and rep2⁺ in MCB gene transcription at the mitotic G1-S boundary in fission yeast. Current Genetics, 40(4), pp. 251-259. (doi: 10.1007/s002940100248)

Abstract

In this paper, we describe the transcription profile of a group of genes at the G1-S boundary of fission yeast in synchronously dividing mitotic cells, under a variety of different conditions. This transcription profile is unaffected in cells where either cdc10⁺ or cdc10-C4 are constitutively overexpressed. In contrast, overexpression of either rep1⁺ or rep2⁺ results in constitutive expression of MCB-regulated genes, suggesting that these polypeptides have important regulatory properties in controlling MCB transcription. Finally, we examine the pattern of MCB-regulated transcription in cells where the G1 period is extended. Surprisingly, we find that the wee1-50 mutation causes MCB transcription throughout the cell cycle, whereas cells re-fed after nitrogen starvation have normal expression patterns. The implications of these observations for understanding MCB-regulated transcription are discussed.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McInerny, Dr Chris and White, Dr Stewart
• Authors: White, S., Khaliq, F., Sotiriou, S., and McInerny, C.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Current Genetics
• Journal Abbr.: Curr. Genet.
• ISSN: 0172-8083
• ISSN (Online): 1432-0983