Bactericidal effects of high-power Nd:YAG laser radiation on Staphylococcus aureus

Yeo, C. B. A., Watson, I. A. , Stewart-Tull, D. E. S., Wardlaw, A. C. and Armstrong, G. N. (1998) Bactericidal effects of high-power Nd:YAG laser radiation on Staphylococcus aureus. Pure and Applied Optics, 7(3), pp. 643-655. (doi: 10.1088/0963-9659/7/3/020)

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The effect of laser radiation on <i>Staphylococcus aureus</i> 6571 (Oxford strain) was studied with high-power Nd:YAG laser radiation between 50 and 300 W. A range of laser pulse repetition frequencies (PRF) from 5 to 30 Hz, with a combination of pulse energies from 2 to 30 J were applied; this covered a range of energy densities from 800 to 2700 J cm<sup>−2</sup>. The area of inactivation of <i>S. aureus</i>, lawned on nutrient agar plates, was quantified as a function of energy density and exposure time. The shortest exposure time which produced an area of inactivation equal to 50% of the beam area was achieved at a PRF of 30 Hz, pulse energy of 10 J, and with an exposure time of 10.75 s; this was equivalent to an applied energy density of 1723 J cm<sup>−2</sup>. No bacterial inactivation was observed at relatively low-power settings for PRF, pulse energies and exposure time of: 20 Hz, 3 J and 34 s; 25 Hz, 2 J and 45 s and 30 Hz, 2 J and 35 s, respectively. These results shows that pulse energy, PRF and exposure time are important criteria when considering inactivation of micro-organisms by laser radiation.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Watson, Dr Ian and Wardlaw, Prof Alastair and Stewart-Tull, Prof Duncan
Authors: Yeo, C. B. A., Watson, I. A., Stewart-Tull, D. E. S., Wardlaw, A. C., and Armstrong, G. N.
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:Pure and Applied Optics
Publisher:IOP Publishing Ltd

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