Eps8 controls Src- and FAK-dependent phenotypes in squamous carcinoma cells

Schoenherr, C., Serrels, B., Proby, C., Cunningham, D. L., Findlay, J. E., Baillie, G. S. , Heath, J. K. and Frame, M. (2014) Eps8 controls Src- and FAK-dependent phenotypes in squamous carcinoma cells. Journal of Cell Science, 127(24), pp. 5303-5316. (doi:10.1242/jcs.157560)

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Abstract

Eps8 is an actin regulatory scaffold protein whose expression is increased in squamous cell carcinoma (SCC) cells. It forms a complex with both focal adhesion kinase (FAK, also known as PTK2) and Src in SCC cells derived from skin carcinomas induced by administration of the chemical DMBA followed by TPA (the DMBA/TPA model). Here, we describe two new roles for Eps8. Firstly, it controls the spatial distribution of active Src in a FAK-dependent manner. Specifically, Eps8 participates in, and regulates, a biochemical complex with Src and drives trafficking of Src to autophagic structures that SCC cells use to cope with high levels of active Src when FAK is absent. Secondly, when FAK is expressed in SCC cells, thereby meaning active Src becomes tethered at focal adhesion complexes, Eps8 is also recruited to focal adhesions and is required for FAK-dependent polarization and invasion. Therefore, Eps8 is a crucial mediator of Src- and FAK-regulated processes; it participates in specific biochemical complexes and promotes actin re-arrangements that determine the spatial localization of Src, and modulates the functions of Src and FAK during invasive migration.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Baillie, Professor George and Findlay, Mrs Jane and Frame, Prof Margaret and Serrels, Dr Bryan
Authors: Schoenherr, C., Serrels, B., Proby, C., Cunningham, D. L., Findlay, J. E., Baillie, G. S., Heath, J. K., and Frame, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Journal of Cell Science
Publisher:Company of Biologists
ISSN:0021-9533
ISSN (Online):1477-9137
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Journal of Cell Science 127(24):5303-5316
Publisher Policy:Reproduced under a Creative Commons License
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