Cell proliferation and migration inside single cell arrays

Chanasakulniyom, M., Glidle, A. and Cooper, J. M. (2015) Cell proliferation and migration inside single cell arrays. Lab on a Chip, 15(1), pp. 208-215. (doi:10.1039/C4LC00774C)

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Abstract

Cell proliferation and migration are fundamental processes in determining cell and tissue behaviour. In this study we show the design and fabrication of a new single cell microfluidic structure, called a “vertically integrated array” or “VIA” trap to explore quantitative functional assays including single cell attachment, proliferation and migration studies. The chip can be used in a continuous (flow-through) manner, with a continuous supply of new media, as well as in a quiescent mode. We show the fabrication of the device, together with the flow characteristics inside the network of channels and the single cell traps. The flow patterns inside the device not only facilitate cell trapping, but also protect the cells from mechanical flow-induced stress. MDA-MB-231 human breast cancer cells were used to study attachment and detachment during the cell cycle as well as explore the influences of the chemokine SDF-1 (enabling the quantification of the role of chemokine gradients both on pseudopod formation and directional cell migration).

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Chanasakulniyom, Miss Mayuree and Glidle, Dr Andrew and Cooper, Professor Jonathan
Authors: Chanasakulniyom, M., Glidle, A., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:R S C Publications
ISSN:1473-0197
ISSN (Online):1473-0189
Copyright Holders:Copyright © 2015 The Royal Society of Chemistry
First Published:First published in Lab On a Chip 15(1):208-215
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
553521Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and WellbeingJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/I017887/1ENG - BIOMEDICAL ENGINEERING