Plasma steroid profiling and response to trophins to illustrate intra-adrenal dynamics

McManus, F., Fraser, R., Davies, E. , Connell, J.M.C. and Freel, E.M. (2015) Plasma steroid profiling and response to trophins to illustrate intra-adrenal dynamics. Journal of Endocrinology, 224, pp. 149-157. (doi: 10.1530/JOE-14-0561)

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Abstract

The importance of corticosteroids in cardiovascular and other chronic disease is recognised. In addition, plasma steroid precursor: product ratios are useful and convenient indirect indicators of efficiency of key steroidogenic enzymes (aldosterone synthase, 11β-hydroxylase and 17α-hydroxylase). The use of liquid chromatography tandem mass spectrometry (LCMS/MS) has enabled measurement of numerous corticosteroid compounds simultaneously. However, normal responses to trophins and variation in salt intake are not well described. This study examined these parameters in a large group of healthy volunteers. 60 normotensive volunteers were recruited and underwent infusion of angiotensin II and ACTH, following low and high salt diet. Plasma steroid measurements at baseline and 30 minutes after infusion of trophin were obtained by LCMS. As expected, plasma mineralocorticoids rose in response to salt restriction and were suppressed with salt loading, ACTH infusion increased all corticosteroids, while AngII increased mineralocorticoids and suppressed glucocorticoid production. ACTH increased S: F but decreased DOC: B, thus the S: F ratio is a more appropriate index of 11β-hydroxylase efficiency. The B: F ratio increased following ACTH treatment and salt restriction. A larger proportion of plasma B than generally accepted may be derived from the zona glomerulosa and this ratio may be most informative of 17α-hydroxylase activity in salt replete subjects. Although DOC: aldosterone, B: aldosterone and 18-hydroxyB: aldosterone should provide indices of aldosterone synthase efficiency, responses of individual compounds to trophins suggest that none accurately reflect this. Based on these data, aldosterone synthase activity is most accurately reflected by aldosterone concentration alone.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Davies, Professor Eleanor and Fraser, Prof Robert and McManus, Dr Frances
Authors: McManus, F., Fraser, R., Davies, E., Connell, J.M.C., and Freel, E.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of Endocrinology
Publisher:BioScientifica Ltd.
ISSN:0022-0795
ISSN (Online):1479-6805
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