Latosinska, A., Frantzi, M., Mullen, W. , Vlahou, A. and Makridakis, M. (2014) Targeting the proteome of cellular fractions: focus on secreted proteins. Methods in Molecular Biology, 1243, pp. 29-41. (doi: 10.1007/978-1-4939-1872-0_2)
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Publisher's URL: http://dx.doi.org/10.1007/978-1-4939-1872-0_2
Abstract
The high complexity of the total cellular proteome underscores the need for a more targeted investigation of particular subcellular fractions as a means to detect the changes at the level of low abundance proteins. However, this approach requires the application of an enrichment strategy. In this chapter, we present the protocols, which have been used for the analysis of secretome from cell lines, targeting the investigation of protein expression changes.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Mullen, Dr Bill |
| Authors: | Latosinska, A., Frantzi, M., Mullen, W., Vlahou, A., and Makridakis, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | Methods in Molecular Biology |
| Publisher: | Humana Press |
| ISSN: | 1064-3745 |
| ISSN (Online): | 1940-6029 |
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