Targeting the proteome of cellular fractions: focus on secreted proteins

Latosinska, A., Frantzi, M., Mullen, W. , Vlahou, A. and Makridakis, M. (2014) Targeting the proteome of cellular fractions: focus on secreted proteins. Methods in Molecular Biology, 1243, pp. 29-41. (doi: 10.1007/978-1-4939-1872-0_2)

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Publisher's URL: http://dx.doi.org/10.1007/978-1-4939-1872-0_2

Abstract

The high complexity of the total cellular proteome underscores the need for a more targeted investigation of particular subcellular fractions as a means to detect the changes at the level of low abundance proteins. However, this approach requires the application of an enrichment strategy. In this chapter, we present the protocols, which have been used for the analysis of secretome from cell lines, targeting the investigation of protein expression changes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mullen, Dr Bill
Authors: Latosinska, A., Frantzi, M., Mullen, W., Vlahou, A., and Makridakis, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Methods in Molecular Biology
Publisher:Humana Press
ISSN:1064-3745
ISSN (Online):1940-6029

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