Exploiting genetic variation to discover genes involved in important disease phenotypes

Capewell, P. , Cooper, A., Clucas, C. , Weir, W. , Vaikkinen, H., Morrison, L., Tait, A. and MacLeod, A. (2015) Exploiting genetic variation to discover genes involved in important disease phenotypes. Methods in Molecular Biology, 1201, pp. 91-107. (doi:10.1007/978-1-4939-1438-8_5) (PMID:25388109)

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Abstract

Elucidating the underlying genetic determinants of disease pathology is still in the early stages for many pathogenic parasites. There have, however, been a number of advances in which natural genetic diversity has been successfully utilized to untangle the often complex interactions between parasite and host. In this chapter we discuss various methods capable of exploiting this natural genetic variation to determine genes involved in phenotypes of interest, using virulence in the pathogenic parasite Trypanosoma brucei as a case study. This species is an ideal system to benefit from such an approach as there are several well-characterized laboratory strains; the parasite undergoes genetic exchange in both the field and the laboratory, and is amenable to efficient reverse genetics and RNAi.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Dr Anneli and MacLeod, Professor Annette and Morrison, Dr Liam and Tait, Professor Andy and Weir, Dr Willie and Clucas, Dr Caroline and Capewell, Dr Paul
Authors: Capewell, P., Cooper, A., Clucas, C., Weir, W., Vaikkinen, H., Morrison, L., Tait, A., and MacLeod, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Methods in Molecular Biology
Publisher:Springer New York
ISSN:1064-3745
ISSN (Online):1940-6029

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