Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids

Stalmach, A. , Husi, H., Mosbahi, K., Albalat, A., Mullen, W. and Mischak, H. (2015) Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids. Methods in Molecular Biology, 1243, pp. 187-205. (doi: 10.1007/978-1-4939-1872-0_11)

Abstract

Proteomic biomarkers hold the promise of enabling assessment of patients according to a pathological condition aiming at improvements in diagnosis, prognosis, in general clinical patient management. Capillary electrophoresis coupled to an electrospray ionization time-of-flight mass spectrometer (CE-MS) allows the detection of thousands of small proteins and peptides in various biofluids, in a single, reproducible and time-limited step, enabling the simultaneous comparison of multiple individual proteins and peptides in biomarker discovery, but also in clinical applications. The reliability of the CE-MS platform, together with the use of a validated approach for data processing and mining is, to date, the most advanced technique for biomarker discovery of clinical significance. In this chapter, we report on the materials, methods and protocols used for CE-MS-based clinical proteomics allowing the reproducible profiling of biofluids.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mosbahi, Dr Khedidja and Stalmach, Dr Angelique and Albalat, Dr Amaya and Mullen, Dr Bill and Husi, Dr Holger and Mischak, Professor Harald
• Authors: Stalmach, A., Husi, H., Mosbahi, K., Albalat, A., Mullen, W., and Mischak, H.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Methods in Molecular Biology
• Publisher: Springer New York
• ISSN: 1064-3745