Doppler imaging of spontaneously occurring feline diastolic dysfunction

Hirschberg, S., Koffas, H., Adolah-Abram, D., Dukes-McEwan, J., Corcoran, B., French, A., Simpson, K. and Ohad, D. (2008) Doppler imaging of spontaneously occurring feline diastolic dysfunction. Israel Journal of Veterinary Medicine, 63(3), p. 91.

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Abstract

Two approaches to the synthesis of biotinylated derivatives of the stress-activated protein kinase (SAPK) pathway activator anisomycin have been investigated. Attachment of the biotin moiety to the central core was achieved either through the use of a classical displacement reaction on a.-halo carbonyl derivatives of biotin or through a copper(I)-catalyzed 1,3-dipolar Huisgen cycloaddition ("click") coupling of biotinylated azides to propargyl-marked analogues of anisomycin. In each case, the resultant N-linked molecular probes were found to be active in SAPK pathway immunoblot assays, while their O-linked counterparts were inactive. However, in sharp contrast to the classical coupling approach which results in low coupling yields, the aqueous "click" coupling process was found to deliver high yields of biotinylated probes, making it the conjugation method of choice. A survey of the available methods for the addition of a propargyl marker onto a range of chemical functionalities strongly suggests that this copper(I)-catalyzed 1,3-dipolar Huisgen cycloaddition approach to biotinylation may be generally applied.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:French, Professor Anne
Authors: Hirschberg, S., Koffas, H., Adolah-Abram, D., Dukes-McEwan, J., Corcoran, B., French, A., Simpson, K., and Ohad, D.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Israel Journal of Veterinary Medicine
Publisher:Israel Veterinary Medical Association
ISSN:0334-9152
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