An investigation of the breadth of neutralising antibody response in cats naturally infected with feline immunodeficiency virus

Beczkowski, P., Logan, N., McMonagle, E., Litster, A., Willett, B. J. and Hosie, M. J. (2015) An investigation of the breadth of neutralising antibody response in cats naturally infected with feline immunodeficiency virus. Journal of General Virology, 96(3), pp. 671-680. (doi:10.1099/vir.0.071522-0) (PMID:25395594) (PMCID:PMC4336861)

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Abstract

Neutralising antibodies (NAbs) are believed to comprise an essential component of the protective immune response induced by vaccines against FIV and HIV infections. However, relatively little is known about the role of NAbs in controlling FIV infection and subsequent disease progression. Here we present studies examining the neutralisation of HIV-luciferase pseudotypes bearing homologous and heterologous FIV Envs (n=278) by sequential plasma samples collected at 6 month intervals from naturally infected cats (n=38) over a period of 18 months. We evaluated the breadth of the NAb response against non-recombinant homologous and heterologous clade A and clade B viral variants as well as recombinants and assessed the results, testing for evidence of an association between the potency of the NAb response and the duration of infection, CD4 T lymphocyte numbers, health status and survival times of the infected cats. Neutralisation profiles varied significantly between FIV infected cats and strong autologous neutralisation, assessed using luciferase based in vitro assays, did not correlate with the clinical outcome. No association was observed between strong NAb responses and either improved health status or increased survival time of infected animals, implying that other protective mechanisms are likely to be involved. Similarly, no correlation was observed between the development of autologous NAbs and the duration of infection. Furthermore, cross-neutralising antibodies were evident in only a small proportion (13%) of cats.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Willett, Professor Brian and Beczkowski, Dr Pawel and McMonagle, Mrs Elizabeth and Logan, Miss Nicola
Authors: Beczkowski, P., Logan, N., McMonagle, E., Litster, A., Willett, B. J., and Hosie, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Journal of General Virology 96(3):671-680
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
509331Quasispecies evolution in the progression of FIV infectionMargaret HosieWellcome Trust (WELLCOME)088486/Z/09ZMVLS III - CENTRE FOR VIRUS RESEARCH
471561The virus-receptor interaction in lentiviral pathogenesis and immunityBrian WillettWellcome Trust (WELLCOME)084272/Z/07/ZMVLS III - CENTRE FOR VIRUS RESEARCH