The host metabolite D-serine contributes to bacterial niche specificity through gene selection

Connolly, J. P.R., Goldstone, R. J., Burgess, K., Cogdell, R. J. , Beatson, S. A., Vollmer, W., Smith, D. G.E. and Roe, A. (2015) The host metabolite D-serine contributes to bacterial niche specificity through gene selection. ISME Journal, 9, pp. 1039-1051. (doi:10.1038/ismej.2014.242) (PMID:25526369) (PMCID:PMC4366372)

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Abstract

Escherichia coli comprise a diverse array of both commensals and niche-specific pathotypes. The ability to cause disease results from both carriage of specific virulence factors and regulatory control of these via environmental stimuli. Moreover, host metabolites further refine the response of bacteria to their environment and can dramatically affect the outcome of the host–pathogen interaction. Here, we demonstrate that the host metabolite, D-serine, selectively affects gene expression in E. coli O157:H7. Transcriptomic profiling showed exposure to D-serine results in activation of the SOS response and suppresses expression of the Type 3 Secretion System (T3SS) used to attach to host cells. We also show that concurrent carriage of both the D-serine tolerance locus (dsdCXA) and the locus of enterocyte effacement pathogenicity island encoding a T3SS is extremely rare, a genotype that we attribute to an ‘evolutionary incompatibility’ between the two loci. This study demonstrates the importance of co-operation between both core and pathogenic genetic elements in defining niche specificity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Roe, Professor Andrew and Beatson, Dr Scott and Burgess, Dr Karl and Cogdell, Professor Richard and Connolly, Dr James and Goldstone, Mr Robert and Smith, Professor David
Authors: Connolly, J. P.R., Goldstone, R. J., Burgess, K., Cogdell, R. J., Beatson, S. A., Vollmer, W., Smith, D. G.E., and Roe, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:ISME Journal
Publisher:Nature Publishing Group
ISSN:1751-7362
ISSN (Online):1751-7370
Copyright Holders:Copyright © 2015 International Society for Microbial Ecology
First Published:First published in ISME Journal 9:1039-1051
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
580941MRC Doctoral Training Grant 2011-2015Mary Beth KneafseyMedical Research Council (MRC)MR/J50032X/1VICE PRINCIPAL RESEARCH & ENTERPRISE