Dynamic acoustic field activated cell separation (DAFACS)

Skotis, G.D., Cumming, D.R.S. , Roberts, J.N. , Riehle, M.O. and Bernassau, A.L. (2015) Dynamic acoustic field activated cell separation (DAFACS). Lab on a Chip, 2015(3), pp. 802-810. (doi:10.1039/C4LC01153H) (PMID:25474444)

Skotis, G.D., Cumming, D.R.S. , Roberts, J.N. , Riehle, M.O. and Bernassau, A.L. (2015) Dynamic acoustic field activated cell separation (DAFACS). Lab on a Chip, 2015(3), pp. 802-810. (doi:10.1039/C4LC01153H) (PMID:25474444)

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Abstract

Advances in diagnostics, cell and stem cell technologies drive the development of application-specific tools for cell and particle separation. Acoustic micro-particle separation offers a promising avenue for highthroughput, label-free, high recovery, cell and particle separation and isolation in regenerative medicine. Here, we demonstrate a novel approach utilizing a dynamic acoustic field that is capable of separating an arbitrary size range of cells. We first demonstrate the method for the separation of particles with different diameters between 6 and 45 μm and secondly particles of different densities in a heterogeneous medium. The dynamic acoustic field is then used to separate dorsal root ganglion cells. The shearless, label-free and low damage characteristics make this method of manipulation particularly suited for biological applications. Advantages of using a dynamic acoustic field for the separation of cells include its inherent safety and biocompatibility, the possibility to operate over large distances (centimetres), high purity (ratio of particle population, up to 100%), and high efficiency (ratio of separated particles over total number of particles to separate, up to 100%).

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cumming, Professor David and Bernassau, Dr Anne and Roberts, Dr Jemma and Riehle, Dr Mathis
Authors: Skotis, G.D., Cumming, D.R.S., Roberts, J.N., Riehle, M.O., and Bernassau, A.L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Copyright Holders:Copyright © 2014 The Royal Society of Chemistry
First Published:First published in Lab on a Chip 2015(3):802-810
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
654251High performance acoustic particle sorting and sensingAnne BernassauRoyal Society (ROYSOC)RG130493ENG - ENGINEERING ELECTRONICS & NANO ENG