Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L.

Braceland, M., McLoughlin, M.F., Tinsley, J., Wallace, C., Cockerill, D., McLaughlin, M. and Eckersall, P.D. (2015) Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L. Journal of Fish Diseases, 28(9), pp. 821-831. (doi:10.1111/jfd.12296) (PMID:25168106)

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Abstract

Diseases which cause skeletal muscle myopathy are some of the most economically damaging diseases in Atlantic salmon, Salmo salar L., aquaculture. Despite this, there are limited means of assessing fish health non-destructively. Previous investigation of the serum proteome of Atlantic salmon, Salmo salar L., during pancreas disease (PD) has identified proteins in serum that have potential as biomarkers of the disease. Amongst these proteins, the enzyme enolase was selected as the most viable for use as a biomarker of muscle myopathy associated with PD. Western blot and immunoassay (ELISA) validated enolase as a biomarker for PD, whilst immunohistochemistry identified white muscle as the source of enolase. Enolase was shown to be a specific marker for white muscle myopathy in salmon, rising in serum concentration significantly correlating with pathological damage to the tissue.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McLaughlin, Dr Mark and Braceland, Mr Mark and Eckersall, Professor David
Authors: Braceland, M., McLoughlin, M.F., Tinsley, J., Wallace, C., Cockerill, D., McLaughlin, M., and Eckersall, P.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Fish Diseases
Publisher:Wiley
ISSN:0140-7775
ISSN (Online):1365-2761
Published Online:29 August 2014
Copyright Holders:Copyright © 2014 John Wiley & Sons Ltd.
First Published:First published in the Journal of Fish Diseases 38(9):821-831
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
530451Genotyping and Phenotyping Biomarkers of the Pathological Response to Pancreas Disease in the Atlantic Salmon.Peter EckersallBiotechnology and Biological Sciences Research Council (BBSRC)BB/I532837/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED